Cell, Gene and Therapy Journal

Peer-reviewed multi-disciplinary and Open Access journal accepts scientifically rigorous research, regardless of novelty

Introduction

Journal of Cell, Gene and Therapy is a peer-reviewed multi-disciplinary and Open Access journal accepts scientifically rigorous research, regardless of novelty. CGT’s broad scope provides a platform to publish basic and clinic research, including interdisciplinary and replication studies as well as negative results. The journal’s publication criteria are based on high ethical standards and the rigor of the methodology and conclusions reported. Specific scientific topics of interest to the journal include, but are not limited to:

  • gene and cell study
  • stem cell therapy
  • Genetics
  • pharmaceutics
  • medicinal biochemistry
  • clinical investigation
  • biotechnology
  • nanotechnology
  • clinical trial.

About the Journal

Journal of Cell، Gene and Therapy (CGT) is a peer-reviewed multi-disciplinary and Open Access journal accepts scientifically rigorous research which is edited by a scientific editorial board.

CGT’s broad scope provides a platform to publish basic and clinic research, including interdisciplinary and replication studies as well as negative results. Scientific topics of interest to the journal include, but are not limited to: gene and cell study, stem cell therapy, Genetics, pharmaceutics, medicinal biochemistry, clinical investigation, biotechnology, nanotechnology, and clinical trials.

CGT released the first issue on April the first 2019 and the second issue is going to be released in July 2019 as well.

We would be very delighted to receive your Original papers, Review Articles, Short communications, Case reports, and Scientific Letters to the Editor on the above-mentioned research areas and we are able to evaluate the received manuscripts and publish them quiet shortly.

Scope

We will also consider the following article types:

  • Systematic reviews. We consider publishing systematic reviews only if the methods ensure the comprehensive and unbiased sampling of existing literature.
  • Submissions describing methods, software, databases, or other tools. We consider submissions describing methods, software, databases, or other tools if they follow the appropriate reporting guidelines.
  • Qualitative research. We consider publishing qualitative research only if it adheres to appropriate study design and reporting guidelines.
  • Clinical study. We consider publishing clinical reports, such as case report, pre-clinical study, clinical trials which are based on the ethical procedures.
  • Studies reporting negative results
Medical News

Modified CRISPR-Cas9 gene editing scissors are enabling researchers to make alterations to the genetic material of single-cell organisms that are indistinguishable from natural mutations. This method is making it possible to develop a (harmless) experimental live vaccine for the widespread parasite Toxoplasma gondii.

Around a third of the world's population carries Toxoplasma gondii, a parasite that puts people with a weakened immune system at risk and can trigger malformations in the womb. The single-celled pathogen also leads to economic losses in agriculture, with toxoplasmosis increasing the risk of abortion among sheep, for example.

A comprehensive study involving more than 250,000 women, shows that oral contraceptive use protects against ovarian and endometrial cancer. The protective effect remains for several decades after discontinuing the use.

A comprehensive study from Uppsala University, involving more than 250,000 women, shows that oral contraceptive use protects against ovarian and endometrial cancer. The protective effect remains for several decades after discontinuing the use. The study is published in the journal Cancer Research.

Researchers have discovered a compound capable of pushing through barriers used by Gram-negative bacteria to resist antibiotics, damaging the bugs and preventing them from spreading

As scientists around the globe wage war against a novel, deadly virus, one University of Colorado Boulder lab is working on new weapons to battle a different microbial threat: a rising tide of antibiotic-resistant bacteria which, if left unchecked, could kill an estimated 10 million people annually by 2050.

Researchers provide comprehensive molecular characterization of head and neck cancers

Researchers provide comprehensive molecular characterization of head and neck cancers

Hundreds of cancer patients have benefitted from using computer algorithms to manage their symptoms and improve their wellbeing in a unique UK trial.

The early stage colorectal, breast or gynecological cancer patients took part in the trial of the eRAPID system, developed by the University of Leeds, which allowed them to report online symptoms from home and receive instant advice on whether to self-manage or seek medical attention.

Authors Guidlines

Submit Your Manuscript

For more information about submitting to CGT, read our checklist for getting started and our guidelines for preparing a submission.

 

MANUSCRIPT PREPARATION

Manuscripts should be prepared in accordance with the “Uniform Requirements for Manuscripts Submission to Cell, Gene and Therapy (CGT) ” proclaimed by the International Committee of Medical Journal Editors. Language. Manuscripts should be in English (either British or American spelling). The past tense should be used throughout in describing the results, and the present tense in referring to previously established and generally accepted results. Authors who are unsure of correct English editing should have their manuscript checked by those proficient in the language; manuscripts that are deficient in this respect may be returned to the author for revision before scientific review.

 

Type setting

Manuscripts must be typewritten in Times New Roman with a font size of 12 points, double-spaced (including References, Tables and Figure legends) with 2.5 cm margins from each side. All pages should be numbered consecutively at the bottom starting with the title page. Length. The maximum length for describing methods, full length papers, reviews an Clinical study (including tables, figures and references) should not exceed 3000, 4500,  6000  and 4500 words, respectively. Any repetition of information in the text and illustrations and excessively list of references must be avoided.

 

GENERAL ARRANGEMENT OF PAPERS

Title

Titles must be concise and informative. The title should be followed by the authors' full (first, middle and last) names and their affiliations (identified by use of Arabic numbers (1, 2, 3, etc.) in superscript format next to the authors’names.

 

Running Title

The text should include a running title of no more than 30 characters including spaces.

 

Footnotes

The name and full postal address, telephone, fax and E-mail number of corresponding author should be provided in a footnote.

 

Abbreviations

 The Journal publishes a standard list of abbreviations at the beginning of every issue. These

standard abbreviations do not need to be spelled out within paper. However, non-standard and undefined abbreviations used five or more times should be listed in the footnote. Abbreviations should be defined where first mentioned in the text. Do not use abbreviations in the title, but they can be used in the Figures and Tables with their description in the figure legend or table footnote.

 

Abstract

 Abstract should follow the title (excluding the authors’ names) on the second page. Abstracts must be written in a structured (Background, Methods, Results, and Conclusion) format, at a maximum of 150 or 250 words for short communications and full papers, respectively. Inclusion of  references must be avoided in the abstracts.

 

Keywords

Three to five keywords for indexing should be included at the foot of the abstract chosen from the Medical Subject Headings (MeSH).

 

Introduction

This should contain a description of the problem under investigation and a brief survey of the

existing literature on the subject.

Materials and Methods

Sufficient details must be provided to allow for the reproduction of the work. Complete

and accurate names of materials, including chemical and organisms, should be specified followed by the suppliers’ information (in parenthesis). System International (SI) units and symbols must be used.

 

Results

 This section should concisely describe the rationale of the investigation and its outcomes. Data should not be replicated in the text, tables or figure.

 

Discussion

 This section should relate the results to the previous pertinent reports report and provide potential interpretations.

 

Acknowledgments

Grant support should be included in this section. It may also include the expression of

gratitude towards contributors, not included in the authors’ list.

 

Ethical Guidelines

Animal and human experimental studies must follow the international guidelines and declarations as well as regulations enforced by the local ethical committees of the countries where the research is done. Experiments on human individuals must be performed after obtaining a signed written informed consent form the subject or his/her

guardian. CGT reserves the right to reject papers based on ethical considerations when the scientific value of the

research does not justify the methods used in the study

Open Access

CGT facilitate open access – namely, free immediate access to, and unrestricted reuse of, original works of all types.

Publication Fees

CGT publication is free.

Contact

Visit the Contact page for details about whom to contact with different queries.

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Our Team

Dr. Saeed Shahbeigi

Chairman

“Neurologist and Neuroimmunologist, Brain Mapping Research Center, Shahid Beheshti University of Medical Sciences, Neuroscience Research Center, Iran University of Medical Sciences, President of Stem cell Association of Tehran-Iran”

Dr. Saeed Shahbeigi Chairman

Dr. Abolfazl Movafagh

Editor-in-chief

“Head of Department of Medical Genetics of Shahid Beheshti Medical University. Vice President society of Medical Genetics Department”

Dr. Abolfazl Movafagh Editor-in-chief

Neda Mansouri

Internal Manager

“Master of Medical Genetics”
from Shahid Beheshti University of Medical Science, Faculty of Medicine

Dr. Neda Mansouri Internal Manager

Editorial Board

  • Chairman

  • Dr Saeed Shahbeigi

    Neurologist and Neuroimmunologist, Brain Mapping Research Centre, Shahid Beheshti University of Medical Sciences, Neuroscience Research Centre, Iran University of Medical Sciences, President of Stem cell Association of Tehran-Iran

  • Editor-in-Chief

  • Dr. Abolfazl Movafagh

    Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran

  • Executive Manager

  • Dr. Neda Mansouri

    Faculty of Medicine, Shahid Beheshti University of Medical Science

  • Advisory Editorial Board

  • Dr. Hesam Abdolhoseinpour
    Dr. Mohammad Ali Arami
    Dr. Sepide Arbabi
    Dr. Ayse Altintas
    Dr. Mandana Moheddin Bonab
    Dr. Gholamreza Bakhshandepour
    Dr. Korosh Gharegozli
    Dr. Mohammad Hassan Heidari
    Dr. Seid Mohammad Taghi Hoseini Tabatabaee
    Dr. Behnam Jamei
    Dr. Mohammad Karimi
    Dr. Ahmad Khatoon Abadi
    Dr. Behnam Mansoori
    Dr. Alireza Mesbahi
    Dr. Abolfazl Movafagh
    Dr. Afshin Moradi
    Dr. Abbas Najari
    Dr. Hafez Norozizadeh
    Dr. Mina Ohadi
    Dr. Hosein pakdaman
    Dr. Ali Akbar Safar Moghadam
    Dr. Sasan Saket
    Dr. Saeed Shahbeigi
    Dr. Mesbah Shams
    Dr. Mojgan Sheikhpour
    Dr. Ali Zali
    Dr. Mahdi Zamani
    Dr. Babak Khalili Hadad

Journal of Cell, Gene and Therapy

peer-reviewed multi-disciplinary and Open Access journal accepts scientifically rigorous research, regardless of novelty

Recent Articles

In silico prediction of structural characteristics profile of ω- conotoxins family; the effect of critical human pathologic temperatures


Khalili Hadad Babak*1
1Dept. of Biological Sciences, Faculty of Sciences, Islamic Azad University, Roudehen Branch, Roudehen, Iran

Conotoxins are neurotoxic peptides synthesized by marine cone snail. They have high potential as pharmaceutical agents, particularly as analgesics for the treatment of neuropathic pain. Well-defined ω-conotoxin family members are GVIA, GVIB, GVIC, MVIIA, MVIIC and MVIID. Some are blockers of the neuromuscular junction and they block calcium channels, it is reasonable to do large-scale synthesis derivative of Conotoxin for pharmaceutical purposes. Because of the short length of the peptides, their synthesis is not hard, but the modifications to enhance the potency have not been successful up to now. The well known ω- conotoxin family (1TT3, 1TTK, 1DW5, 1CNN, 1OMG, 1MVI, 1DW4, 1MVJ, 2KM9 and 1FEO) crystallographic data were obtained from PDB data bank. VMD 1.8.2 was used to draw and analyze the Ramachandran plot after they were being simulated at critical physiologic and pathologic human body temperatures with Monte carlo method in MM+ force filed. The most stable structure in terms of potential energy probability was 1TT3. Except 1DW4 and 1DW5 which did not show any structural changes in terms of gyration radius, there were significant differences between crystallographic data, analyzed physiologic and pathologic structures of other PDB codes. This functionality will lead us toward drug designing new blocker drugs of Neuromascular junction by biologically inspired design drug technology.

Subdural hematoma as a complication of Spinal Anesthesia


Shahbeigi Saeed1, Parian Parisadat*2, Nekutalaban Parham3, keyhanifard majid4
1 Brain Mapping Research Centre, Shahid Beheshti University, Neuroscience Research Centre, Iran University of Medical Sciences.
2 Medical Doctor, Semnan University of Medical Sciences, Semnan, Iran.
3 Student Research committee, Qom university of Med ical Science, Qom, Iran.
4 Neuro_intervention Department, Baghiatollah Hospital

Subdural hematoma (SDH) is a rare but important sequel of spinal anesthesia. We present a case of intracranial SDH in a lady developing headache after Cesarean section. A 34-years-old primigravida at 39 weeks of pregnancy was scheduled as an elective Cesarean section (c/s). She had no significant problems and also the pregnancy was done without any complications. The spinal anesthesia was given at L3-4 level by 25 G Quincke needle. Dural puncture was done well and was atraumatic. Two hours after delivery the headache had begun. That was on the frontal area with no other symptoms such as nausea and vomiting, confusion and loss of consciousness. She received Corticosteroid and then her headaches improved relatively but a few hours after discharge, she developed a frontotemporal headaches, that was became more and more and in about one month, she had headaches almost every day. After two months, she suddenly experienced much more severe headache with the left side paresthesia and numbness. She referred to a hospital. On the admission, the ophthalmological and neurological exams were done. Ophtalmological exams were normal. The brain MRI revealed a small SDH in the right frontal region without pressure effect. The results of her lab data were totally in normal range. We found that the spinal analgesia could be the only possibility of SDH. On the sequential follow up brain MRI, the SDH was completely resolved and her headaches were abolished as well. Conclusion: It is recommended when the patient has a persistent and prolonged headaches or a changed character one after doing lumbar puncture, SDH should be considered as a serious complication, just like the aforementioned case.

Comparison of life quality in pre and posttreatment groups of patients with allergic rhinitis


Mafibordbar Sahar*1, Faghih Hossein1, Fathi Mohammad2
1 Gazvin University of Medical Science, Qazvin, Iran
2 Allergic and clinical Immunologist, Children Growth Research Center, Gazvin University of Medical Sciences, Qazvin, Iran

Introduction: Allergic rhinitis is a common problem in global health so that a quarter of the world population suffers from this disease. Also, the disease has a negative effect on the physical/psychological/ social performance of affected people and greatly affects the quality of life of patients. As a result, studies on the impact of current therapies on improving quality of life are important. Therefore, in this study, we examine the effect of treatment with a nasal spray of fluticasone and montelukast in the quality of life of patients. Methods: 195 patients with allergic rhinitis aged 15-60 years from both sexes who candidates for treatment with nasal fluticasone (50 µg once daily) and montelukast (10mg per night) were randomly selected to examine the quality of life and the relative factors. The patient’s quality of life was measured prior and two weeks after the treatment, using Juniper internationally validated the questionnaire. Results: In the current epidemiological study, women (59.5%) had a higher proportion of people with allergic rhinitis. Additionally, the highest prevalence of allergic rhinitis was seen among housewives (29.7%). The frequency of mild continuous allergic rhinitis (22.6%), intermittent (17.9%), and severe continuous type (15.9%) and intermittent (43.6%) was calculated. The results of this study showed that quality of life in 66.2% of patients was known to have made before any treatment intervention, and 33.8% had moderate-quality life defects. Two weeks after the start of treatment with fluticasone and montelukast, 172 (88.2%) had a mild quality of life defect and 23 (11.8%) had moderate to severe impairment in quality of life. Among the symptoms of nasal congestion, itching, and runny nose, frequent sneezing and pruritus, and runny nose, there was only a significant relationship between itching and runny nose and impaired quality of life after treatment. However, there was no association among the severity of the disease before treatment, age, sex, occupation, or asthma and quality of life after treatment (P> 0.05). In addition, there was a significant difference between sleep disorder before and after treatment with fluticasone and montelukast (P <0.001) Conclusion: The results of this study showed that two-weeks combination therapy with fluticasone and oral montelukast, significantly improved the quality of life and sleep disturbance. Additionally, there was a significant relationship between pruritus and runny nose and impaired quality of life after treatment. This could be due to the improvement of these symptoms after receiving the treatments.

Efficacy of zinc sulfate on common cold in children aged 1-5 years admitted at Javaheri hospital in Tehran


Hematyar Masoumeh*1, Nikta Basim2
1 Associate Professor of Pediatrics, Islamic Azad University Tehran Medical Science
2 General Practitioner, Islamic Azad University Tehran Medical Science

Introduction: Common cold is the most common acute upper respiratory tract disease in children. The efficacy of vitamins and minerals for duration, symptoms and complications of common cold has been evaluated in several studies. This study was performed to determine the efficacy of zinc sulfate on common cold in children aged 1-5 years attending to Javaheri hospital in 2012-2013. Materials and Methods: In this clinical trial study, 112 children with common cold aged 1 to 5 years who were admitted at Javaheri hospital in 2012-2013 were enrolled. They were randomly assigned to receive either conventional treatment including Acetaminophen, Pediatric cold and nasal drop as control group or the aforementioned drugs plus zinc sulfate 1 mg/kg/day for 5 days. The duration of symptoms and rate of complications were compared between two groups. Results: The results revealed that the duration of disease was significantly shorter in case group (P = 0.033). Also the frequency of nasal discharge, sneezing, cough and sinusitis was significantly less in case group (P<0.05). Conclusion: In summary, it can be concluded that zinc sulfate is effective in reducing the symptoms, duration and complications of common cold in children aged from 1-5 years within 24 hours of the disease onset.

Effect of eight weeks DNS exercises quality of life and function in women with multiple sclerosis


Zamani Sima*1, Gnaji Behnaz2, Shahbeigi Saeed3
1 MS. C in sport injuries and corrective exercise, Islamic Azad University, Karaj, Alborz, Iran.
2 Ph. D, department of health and sport medicine, school of physical education, Islamic Azad University, Karaj, Alborz, Iran.
3 Brain mapping Research center, Shahid Beheshti Medical University.

Background: DNS exercises cause stimulating the afresh developmental process of motor nervous system, especially for people with movement problems caused by nervous system's disease through actuating neurodevelopmental locomotor chains based on motor positions of a healthy newborn. Methods: Current study intends to survey this exercise’s effect on 30 women with 0-5.5 EDSS and from 23 to 58 years old. In this research for probing the quality of life factors we used the quality of life questionnaire (MSQOL-54) and for the study of physical function like endurance walking the 6 minutes walking test and as of walking pace the 10 meter ling test was used. Results: Our test results showed t-independent and Mann-Whitney which showed that after 8 weeks of DNS exercises the average of overall quality of life between the control and experimental groups (p=0/001) and their average pace and endurance between the control and experimental groups (p=0/000) is significant difference (α≤0/05). Conclusion: So in conclusion the 8 weeks period of DNS exercises resulted in the quality of life factors, pace and endurance in walking and can be used as a remedial method in quality of life and physical function improvement.

Detecting of STK11 gene nucleotide polymorphisms of the germline in patients with Gastrointestinal cancers


Hosseini Seyed Jamal1, Nazemi Ali1, Hashemi Mehrdad*2, Miri Nargesi Mirsaed1, Sharirfi Sharashout1, Movafagh Abolfazl3,4
1 Department of Biology, The Islamic Azad University-Tonekabon, Iran.
2 Department of Molecular Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran.
3 Department of Medical Genetics, Cancer, Research Center, Shohada, Hospital, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.
4 Cancer Research Center, Shohada, Hospital, School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.

Introduction: High resolution melting (HRM) analysis is a technique to measure the decrease of fluorescing, caused by a discharge of the dye, throughout the DNA thermal melting gradient process. STK11 gene encodes one of the cellular serine-threonine kinase proteins that regulate cellular polarity. It also acts as a tumor suppressor protein. Mutations of the germline in the gene are coincident with Peutz-Jeghers syndrome and the potential of developing a variety of neoplasia. Method: In an experimental study, the genome DNA of 56 patients with digestive system cancer was extracted. Afterward, nucleotide changes over the gene were examined using Real-time PCR and High resolution melting (HRM). Findings: Nucleotide screening using the HRM technique revealed two types of SNP in introns 6 & 7 in 10 patients. Four patients showed homozygous C/T nucleotide changes [cluster id/dsSNP/rs9282860] in intron 6 and six patients showed heterozygous C/G nucleotide changes [cluster id/dsSNP/rs2075607] in intron 7. A comparison of HRM results with sequencing results indicated 100% conformity. Conclusion: Although no mutation was observed in exon section of the gene, primary screening of the STK11 gene to diagnose unknown nucleotide changes of germline and somatic in patients with neoplasia using HRM was feasible, easy, and cost-effective.

Cell Cultured Procedure in Normal Human Lymphocytes for Stem Cell Therapy


Mansouri Neda1, Movafagh Abolfazl*1, Sheikhpour Mojgan2, Moradi Afshin3, Ahadi Mahsa3, Niloufar Safavi4, Heidari Mohammad Hassan5
1 Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2 Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
3 Cancer Research Center, Department of Pathology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4 Department of Medical Bactriology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Abnormalities in mitosis parameter and cycle cell program are announced to be based on both the cell proliferation conditions and the condition of the metaphases of each person to respond to Phytohaemagglutinin (PHA) working condition. The aim of the current research is hence to comparison of mitotic index outcome in the individuals of normal volunteers involved in two different period of culture with effect of PHA. Methods: Comparison of healthy human donors lymphocytes were cultured for 48-h and 72-h and using conventional karyotype technique with minor modification. Results: Mitotic index and cell proliferation frequency were analyzed in the individuals of normal blood donors after preparing of colchcine to cultured metaphases 2 hours before cell fixation at. The metaphases indics at 48-h and 72-h culture period maintained a perfect yield level. Lymphocytes populations in 72-houres laboratory work were more than 48-h cultures (2.73% at 72 hours vs. 1.48% at 48 hours, p ≤ 0.05). Conclusion: The present research indicates significant comparisons for laboratories and individuals to be set up. With effect of these results it may help in time consuming and short time period for patient, as well as fresh suggestion for health care management decision. Reagent, manual, budget, time for diagnosis, and health care fulfillment may resolve of this proposal

Effect of serum ferritin level on pulmonary dysfunction and sensitisation to aeroallergens in paediatric beta thalassemia major


Soheila Kianpour Rad 1*, Seyed Mohammad Fathi 2, Hadi Mousakhani 3
1 Qazvin University of Medical Science, Qazvin, Iran
2 Allergist& Clinical Immunologist ,Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
3 Hematologist & Oncologist,Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

The beta thalassemia major (BTM) is the most severe type of thalassemia. Outlining steps in such health problems are vital, since affected children who need repeated transfusions undergo deposition of iron in their different body tissue, causing various dysfunctions, including lung impairment. 33 BTM patients were examined by Pulmonary Function Tests (PFTs) and also skin Prick test and the serum ferritin level was checked. The study demonstrated that 60.5% of the patients had normal PFTs, 21.33% showed restrictive, 12.12% obstructive pattern and two (6.06%) had the mixed pattern and the predominant pulmonary dysfunction was restrictive. Severity of the restrictive pattern has significant correlation with serum ferritin level but not with age, sex, Body Mass Index (BMI) and duration of transfusion therapy. The majority of Splenectomised (SPL) patients had restrictive pattern. Only 5 patients had positive prick test which shows that BTM did not relate to their positive prick test. Cell, Gene and Therapy, Vol.1, Number 1, April 1st, 2019; 64-72

Remarkable effects of Neptune krill oil and fish oil in combating inflammation and pain in mouse model by effect on TNFα and IL-6


Mojtahed Zadeh-Ardabili Parastoo*1
1Physiology department, Faculty of Medicine, University of Medical Science, Mashhad, Iran

Background: Although inflammation is a reactive to injurious stimuli and considered to be beneficial process in body, but it causes some discomforts, such as pain. Murine dietary which contain appreciable fatty-acids, antioxidants, encourage researchers to focus on their potential therapeutic effects. This study is aimed to examine the analgesic and anti-inflammatory activity of Neptune krill oil (NKO) and fish oil (FO) which are two well-known sources of rich content of n-3 polyunsaturated fatty acids (n-3 PUFAs), mostly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in rodent models. NKO and FO were used at same dose of 500 mg and also balanced at similar doses of EPA: 12 in NKO vs. 12 in FO wt%, DHA: 7 NKO vs. 8 FO wt%. Application of NKO and FO in acetic acid-induced writhing effect, hot plate, and formalin induced test, indicated the nociceptive activity of the two tested drugs in comparison with normal saline. Also, the anti-inflammatory effect of these supplements was confirmed by carrageenan test. Analysis of cytokines levels in the blood samples of the mice after induction inflammation by carrageenan indicated decreased levels of those proteins compared to that in the normal groups. Both tested drugs, effectively could reduce severe inflammation and pain in rodents in comparison with the references drugs (depends on the tests), however, NKO is almost found to be more potent than FO in the most assays.

Study of antioxidant and DNA damage protection activities of different solvent extracts of Cinnamomum cassia


Kianpour Rad Sima*1, Hajiaghaalipour Fatemeh2
1 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
2 Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Cinnamomum cassia bark was sequentially extracted with seven solvents of various polarities. The extracts were studied in vitro for the total phenolic and flavonoid contents and the antioxidant activity of using various assays. Also, the DNA damage protective activity of the plant using comet assay of the extracts was investigated. The acetone extract had the highest FRAP value compared to the other extracts. For DPPH scavenging, the lowest IC50 values belonged to the hexane extract, the IC50 of the chloroform extract was the lowest among the IC50 of the other extracts in superoxide radical scavenging. The ethyl acetate extract had the highest antiradical property by inhibiting hydroxyl radical. The IC50 value obtained for the hexane extract was the lowest compared to the other extracts. The acetone extract had the highest content phenolic and flavonoid compound compared to the other extracts. The acetone extract at 30 µg/ml, protected the mouse fibroblast cell line, 3T3-L1, from DNA damage by 45 %, as estimated by the comet assay. This study provides evidence that different extracts of Cinnamomum cassia bark are potential source of natural antioxidants and the acetone extract which showed the highest phenolic compound has a high DNA damage protective activity.

The Functioning of Stem Cell and Transposable Elements in Nucleic Acid and Protein Mechanism


Shaabanpour Aghamaleki Fatemeh1, Sheikhpour Mojgan2, Kianpour Rad Sima3, Ekramnia Iman4, Keramatinia Aliasghar5, Mehrvar Narjes5, Safavi Naini Niloufar4, Movafagh Abolfazl*4
1. Department of Cellular-Molecular Biology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University. Tehran, Iran
2. Department of Mycobacteriology and Pulmonary Research Microbiology, Research Center Pasteur Institute of Iran.
3. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
4. Department of Medical Genetics, Cancer Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5. Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Transposable elements are mobile genetic units that exhibit broad diversity in their structure and transposition mechanisms. Transposable elements occupy a large fraction of many eukaryotic nucleic acid, protein and their movement and accumulation represent a major force shaping the genes and genomes of almost all organisms. Sequence repeats are an important phenomenon in the human genome, playing important roles in genomic alteration often with phenotypic consequences. The two major types of repeat elements in the human genome are tandem repeats (TRs) including microsatellites, minisatellites, and satellites and transposable elements (TEs). Conclusion: TEs are contributing to genome expansion and alteration not only by transposition but also by generating tandem repeats. Finally, this article highlight how the distinctive biological features of DNA transposons have contributed to shape genome architecture and led to the emergence of genetic innovations in different eukaryotic nucleic acid, protein lineages.

Evaluating the effect of intravenous immunoglubin in neonatal hemolytic jaundice caused by Rh and ABO isoimmunization


Masoumeh Hematyar1, Mehdi Zareian2
1Associate professor of “Pediatrics”, Islamic Azad University, Tehran Medical Branch
2General practitioner, Islamic Azad University, Tehran Medical Branch

History & Background: Several studies have shown that prescribing intravenous immunoglobulin(IVIG) can reduce the severity of neonatal hemolytic jaundice due to Rh and ABO incompatibility as well as the need for exchange transfusion. The present study aimed to evaluate the effect of IVIG in treatment of neonatal hemolytic jaundice caused by Rh and ABO isoimmunization. Methods: This clinical trial study was conducted on 80 newborns with neonatal hemolytic jaundice caused by Rh and ABO isoimmunization hospitalized in the neonatal ward of Mahdieh Hospital in Tehran, Iran from 2017 to 2018. The infants were randomly assigned to case (phototherapy and IVIG) and control (phototherapy) groups. IVIG was prescribed with the dosage of 500mg/kg during 4 hours and which could be repeated for 3 doses, if required. The severity of jaundice, duration of hospitalization for phototherapy , need for exchange transfusion and IVIG complications were studied and recorded in all patients. The data were analyzed using SPSS software, Chi square and independent T-tests. Results: In patients who received both IVIG and phototherapy, the duration of phototherapy and hospitalization as well as the need for exchange transfusion were significantly lower than the control group. No IVIG complications were observed. Conclusion: Prescribing IVIG is an effective and safe treatment that can reduce the severity of jaundice , the need for exchange transfusion and duration of phototherapy in neonatal hemolytic jaundice due to Rh and ABO incompatibility. Cell, Gene and Therapy, Vol.1, Number 1, April 1st, 2019; 24- 28

The Therapeutic Roles of TAZ biomarker [WWTR1] in Cancer


Mahsa Mohammadzadeh1, Mehrdad Hashemi1*, Babak Negahdari2, 3 , Fatemeh Ghaemimanesh2

1 Faculty of advanced sciences and technology, Department of Genetics, Islamic Azad University, Tehran Medical Sciences , Tehran, Iran
2 Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
3 Department of Medical Biotechnology, School of Advanced Science in Medicine, Tehran University of Medical Science, Tehran, Iran

Many studies show the role of TAZ biomarkers in multiple malignancies. This study has focused on the significance of TAZ biomarker (WWTR1) in diagnosis or development of multiple cancers such as lung cancer, ovarian cancer, colorectal cancer, and breast cancer. The use of biomarkers is now a new way of detecting many diseases, such as cancer. This way, for instance, more than 90% of ovarian cancers are diagnosed in their early stage. This research is included in the results of the related articl

Clinical Manifestations of Anti-NMDAR Encephalitis: Western Canadian Case Series and Practical Recommendations for Treatment


Saeed Shahbeigi [Brain Mapping Research Centre, Shahid Beheshti University of Medical Sciences, Neuroscience Research Center, Iran University of Medical Sciences], Amir Ali Sepehry [Faculty of Medicine, Division of Neurology, University of British Columbia, Vancouver, Canada] , Joel Oger [Emeritus Professor of University of British Columbia]

Anti-NMDAR encephalitis is a life threatening yet treatable neuroimmune disease secondary to the presence of autoantibodies. Despite a growing body of literature, this disorder remains under-recognized, due to variable presentations, and limited clinicians familiarity with the condition. Methods: We describe three cases that were diagnosed at Vancouver General Hospital, Canada. Results: Case 1, a 23-year old Caucasian woman presented with low-grade fever, personality changes, and status epilepticus. She had a normal brain MRI and CSF markers. Anti-NMDAR (NR1) was positive in serum. She had bilateral ovarian teratomas. After oophorectomy and immunosuppressive treatments, the NMDAR antibodies became negative and her seizures completely disappeared. Case 2, following delivery, a 27-year Chinese woman rapidly developed sudden significant behavioural changes, and seizure. She had a teratoma and positive NMDAR (NR1) in the CSF and serum. Despite bilateral oophorectomy and receiving high doses of immunosuppression, she has developed severe sequelae. Case 3, a 32-year Philippino-Canadian female developed short-term memory problems, olfactory hallucinations, and orofacial dyskinesia for 2 years. After receiving steroids, PLEX and IVIG, she clinically improved markedly without any sequel. It is important to mention, on the paper we talk about the first and second line of therapy and interestingly a new treatment option called Bortizomib for refractory NMDAR patients to 1st and 2nd line of therapy as well. Conclusion: Based on our experience when the diagnosis of the anti-NMDAR encephalitis is made in the presence of normal brain MRI and CSF the prognosis is better

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