Cell, Gene and Therapy Journal

Peer-reviewed multi-disciplinary and Open Access journal accepts scientifically rigorous research, regardless of novelty

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Study of antioxidant and DNA damage protection activities of different solvent extracts of Cinnamomum cassia

Kianpour Rad Sima*1, Hajiaghaalipour Fatemeh2
1 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
2 Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Cinnamomum cassia bark was sequentially extracted with seven solvents of various polarities. The extracts were studied in vitro for the total phenolic and flavonoid contents and the antioxidant activity of using various assays. Also, the DNA damage protective activity of the plant using comet assay of the extracts was investigated. The acetone extract had the highest FRAP value compared to the other extracts. For DPPH scavenging, the lowest IC50 values belonged to the hexane extract, the IC50 of the chloroform extract was the lowest among the IC50 of the other extracts in superoxide radical scavenging. The ethyl acetate extract had the highest antiradical property by inhibiting hydroxyl radical. The IC50 value obtained for the hexane extract was the lowest compared to the other extracts. The acetone extract had the highest content phenolic and flavonoid compound compared to the other extracts. The acetone extract at 30 µg/ml, protected the mouse fibroblast cell line, 3T3-L1, from DNA damage by 45 %, as estimated by the comet assay. This study provides evidence that different extracts of Cinnamomum cassia bark are potential source of natural antioxidants and the acetone extract which showed the highest phenolic compound has a high DNA damage protective activity.

Keywords : Cinnamomu cassia, Antioxidant, Extraction, Phenolic content, Flavonoid content, DNA protective activity


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