Cell, Gene and Therapy Journal

Peer-reviewed multi-disciplinary and Open Access journal accepts scientifically rigorous research, regardless of novelty

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Cell Cultured Procedure in Normal Human Lymphocytes for Stem Cell Therapy

Mansouri Neda1, Movafagh Abolfazl*1, Sheikhpour Mojgan2, Moradi Afshin3, Ahadi Mahsa3, Niloufar Safavi4, Heidari Mohammad Hassan5
1 Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2 Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
3 Cancer Research Center, Department of Pathology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4 Department of Medical Bactriology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Abnormalities in mitosis parameter and cycle cell program are announced to be based on both the cell proliferation conditions and the condition of the metaphases of each person to respond to Phytohaemagglutinin (PHA) working condition. The aim of the current research is hence to comparison of mitotic index outcome in the individuals of normal volunteers involved in two different period of culture with effect of PHA.
Methods: Comparison of healthy human donors lymphocytes were cultured for 48-h and 72-h and using conventional karyotype technique with minor modification.
Results: Mitotic index and cell proliferation frequency were analyzed in the individuals of normal blood donors after preparing of colchcine to cultured metaphases 2 hours before cell fixation at. The metaphases indics at 48-h and 72-h culture period maintained a perfect yield level. Lymphocytes populations in 72-houres laboratory work were more than 48-h cultures (2.73% at 72 hours vs. 1.48% at 48 hours, p ≤ 0.05).
Conclusion: The present research indicates significant comparisons for laboratories and individuals to be set up. With effect of these results it may help in time consuming and short time period for patient, as well as fresh suggestion for health care management decision. Reagent, manual, budget, time for diagnosis, and health care fulfillment may resolve of this proposal

Keywords : Comparison; Cell proliferation index; 48-h and 72-h cultured lymphocyte; Phytohaemagglutinin (PHA); Normal donor


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