Cell, Gene and Therapy Journal

Peer-reviewed multi-disciplinary and Open Access journal accepts scientifically rigorous research, regardless of novelty

Cell, Gene and Therapy - Vol 1, No 1, Spring 2020

Articles of this journal

Clinical Manifestations of Anti-NMDAR Encephalitis: Western Canadian Case Series and Practical Recommendations for Treatment

Saeed Shahbeigi [Brain Mapping Research Centre, Shahid Beheshti University of Medical Sciences, Neuroscience Research Center, Iran University of Medical Sciences], Amir Ali Sepehry [Faculty of Medicine, Division of Neurology, University of British Columbia, Vancouver, Canada] , Joel Oger [Emeritus Professor of University of British Columbia]

Anti-NMDAR encephalitis is a life threatening yet treatable neuroimmune disease secondary to the presence of autoantibodies. Despite a growing body of literature, this disorder remains under-recognized, due to variable presentations, and limited clinicians familiarity with the condition. Methods: We describe three cases that were diagnosed at Vancouver General Hospital, Canada. Results: Case 1, a 23-year old Caucasian woman presented with low-grade fever, personality changes, and status epilepticus. She had a normal brain MRI and CSF markers. Anti-NMDAR (NR1) was positive in serum. She had bilateral ovarian teratomas. After oophorectomy and immunosuppressive treatments, the NMDAR antibodies became negative and her seizures completely disappeared. Case 2, following delivery, a 27-year Chinese woman rapidly developed sudden significant behavioural changes, and seizure. She had a teratoma and positive NMDAR (NR1) in the CSF and serum. Despite bilateral oophorectomy and receiving high doses of immunosuppression, she has developed severe sequelae. Case 3, a 32-year Philippino-Canadian female developed short-term memory problems, olfactory hallucinations, and orofacial dyskinesia for 2 years. After receiving steroids, PLEX and IVIG, she clinically improved markedly without any sequel. It is important to mention, on the paper we talk about the first and second line of therapy and interestingly a new treatment option called Bortizomib for refractory NMDAR patients to 1st and 2nd line of therapy as well. Conclusion: Based on our experience when the diagnosis of the anti-NMDAR encephalitis is made in the presence of normal brain MRI and CSF the prognosis is better

The Therapeutic Roles of TAZ biomarker [WWTR1] in Cancer

Mahsa Mohammadzadeh1, Mehrdad Hashemi1*, Babak Negahdari2, 3 , Fatemeh Ghaemimanesh2

1 Faculty of advanced sciences and technology, Department of Genetics, Islamic Azad University, Tehran Medical Sciences , Tehran, Iran
2 Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
3 Department of Medical Biotechnology, School of Advanced Science in Medicine, Tehran University of Medical Science, Tehran, Iran

Many studies show the role of TAZ biomarkers in multiple malignancies. This study has focused on the significance of TAZ biomarker (WWTR1) in diagnosis or development of multiple cancers such as lung cancer, ovarian cancer, colorectal cancer, and breast cancer. The use of biomarkers is now a new way of detecting many diseases, such as cancer. This way, for instance, more than 90% of ovarian cancers are diagnosed in their early stage. This research is included in the results of the related articl

Evaluating the effect of intravenous immunoglubin in neonatal hemolytic jaundice caused by Rh and ABO isoimmunization

Masoumeh Hematyar1, Mehdi Zareian2
1Associate professor of “Pediatrics”, Islamic Azad University, Tehran Medical Branch
2General practitioner, Islamic Azad University, Tehran Medical Branch

History & Background: Several studies have shown that prescribing intravenous immunoglobulin(IVIG) can reduce the severity of neonatal hemolytic jaundice due to Rh and ABO incompatibility as well as the need for exchange transfusion. The present study aimed to evaluate the effect of IVIG in treatment of neonatal hemolytic jaundice caused by Rh and ABO isoimmunization. Methods: This clinical trial study was conducted on 80 newborns with neonatal hemolytic jaundice caused by Rh and ABO isoimmunization hospitalized in the neonatal ward of Mahdieh Hospital in Tehran, Iran from 2017 to 2018. The infants were randomly assigned to case (phototherapy and IVIG) and control (phototherapy) groups. IVIG was prescribed with the dosage of 500mg/kg during 4 hours and which could be repeated for 3 doses, if required. The severity of jaundice, duration of hospitalization for phototherapy , need for exchange transfusion and IVIG complications were studied and recorded in all patients. The data were analyzed using SPSS software, Chi square and independent T-tests. Results: In patients who received both IVIG and phototherapy, the duration of phototherapy and hospitalization as well as the need for exchange transfusion were significantly lower than the control group. No IVIG complications were observed. Conclusion: Prescribing IVIG is an effective and safe treatment that can reduce the severity of jaundice , the need for exchange transfusion and duration of phototherapy in neonatal hemolytic jaundice due to Rh and ABO incompatibility. Cell, Gene and Therapy, Vol.1, Number 1, April 1st, 2019; 24- 28

The Functioning of Stem Cell and Transposable Elements in Nucleic Acid and Protein Mechanism

Shaabanpour Aghamaleki Fatemeh1, Sheikhpour Mojgan2, Kianpour Rad Sima3, Ekramnia Iman4, Keramatinia Aliasghar5, Mehrvar Narjes5, Safavi Naini Niloufar4, Movafagh Abolfazl*4
1. Department of Cellular-Molecular Biology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University. Tehran, Iran
2. Department of Mycobacteriology and Pulmonary Research Microbiology, Research Center Pasteur Institute of Iran.
3. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
4. Department of Medical Genetics, Cancer Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5. Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Transposable elements are mobile genetic units that exhibit broad diversity in their structure and transposition mechanisms. Transposable elements occupy a large fraction of many eukaryotic nucleic acid, protein and their movement and accumulation represent a major force shaping the genes and genomes of almost all organisms. Sequence repeats are an important phenomenon in the human genome, playing important roles in genomic alteration often with phenotypic consequences. The two major types of repeat elements in the human genome are tandem repeats (TRs) including microsatellites, minisatellites, and satellites and transposable elements (TEs). Conclusion: TEs are contributing to genome expansion and alteration not only by transposition but also by generating tandem repeats. Finally, this article highlight how the distinctive biological features of DNA transposons have contributed to shape genome architecture and led to the emergence of genetic innovations in different eukaryotic nucleic acid, protein lineages.

Study of antioxidant and DNA damage protection activities of different solvent extracts of Cinnamomum cassia

Kianpour Rad Sima*1, Hajiaghaalipour Fatemeh2
1 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
2 Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Cinnamomum cassia bark was sequentially extracted with seven solvents of various polarities. The extracts were studied in vitro for the total phenolic and flavonoid contents and the antioxidant activity of using various assays. Also, the DNA damage protective activity of the plant using comet assay of the extracts was investigated. The acetone extract had the highest FRAP value compared to the other extracts. For DPPH scavenging, the lowest IC50 values belonged to the hexane extract, the IC50 of the chloroform extract was the lowest among the IC50 of the other extracts in superoxide radical scavenging. The ethyl acetate extract had the highest antiradical property by inhibiting hydroxyl radical. The IC50 value obtained for the hexane extract was the lowest compared to the other extracts. The acetone extract had the highest content phenolic and flavonoid compound compared to the other extracts. The acetone extract at 30 µg/ml, protected the mouse fibroblast cell line, 3T3-L1, from DNA damage by 45 %, as estimated by the comet assay. This study provides evidence that different extracts of Cinnamomum cassia bark are potential source of natural antioxidants and the acetone extract which showed the highest phenolic compound has a high DNA damage protective activity.

Remarkable effects of Neptune krill oil and fish oil in combating inflammation and pain in mouse model by effect on TNFα and IL-6

Mojtahed Zadeh-Ardabili Parastoo*1
1Physiology department, Faculty of Medicine, University of Medical Science, Mashhad, Iran

Background: Although inflammation is a reactive to injurious stimuli and considered to be beneficial process in body, but it causes some discomforts, such as pain. Murine dietary which contain appreciable fatty-acids, antioxidants, encourage researchers to focus on their potential therapeutic effects. This study is aimed to examine the analgesic and anti-inflammatory activity of Neptune krill oil (NKO) and fish oil (FO) which are two well-known sources of rich content of n-3 polyunsaturated fatty acids (n-3 PUFAs), mostly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in rodent models. NKO and FO were used at same dose of 500 mg and also balanced at similar doses of EPA: 12 in NKO vs. 12 in FO wt%, DHA: 7 NKO vs. 8 FO wt%. Application of NKO and FO in acetic acid-induced writhing effect, hot plate, and formalin induced test, indicated the nociceptive activity of the two tested drugs in comparison with normal saline. Also, the anti-inflammatory effect of these supplements was confirmed by carrageenan test. Analysis of cytokines levels in the blood samples of the mice after induction inflammation by carrageenan indicated decreased levels of those proteins compared to that in the normal groups. Both tested drugs, effectively could reduce severe inflammation and pain in rodents in comparison with the references drugs (depends on the tests), however, NKO is almost found to be more potent than FO in the most assays.

Effect of serum ferritin level on pulmonary dysfunction and sensitisation to aeroallergens in paediatric beta thalassemia major

Soheila Kianpour Rad 1*, Seyed Mohammad Fathi 2, Hadi Mousakhani 3
1 Qazvin University of Medical Science, Qazvin, Iran
2 Allergist& Clinical Immunologist ,Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
3 Hematologist & Oncologist,Children Growth Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

The beta thalassemia major (BTM) is the most severe type of thalassemia. Outlining steps in such health problems are vital, since affected children who need repeated transfusions undergo deposition of iron in their different body tissue, causing various dysfunctions, including lung impairment. 33 BTM patients were examined by Pulmonary Function Tests (PFTs) and also skin Prick test and the serum ferritin level was checked. The study demonstrated that 60.5% of the patients had normal PFTs, 21.33% showed restrictive, 12.12% obstructive pattern and two (6.06%) had the mixed pattern and the predominant pulmonary dysfunction was restrictive. Severity of the restrictive pattern has significant correlation with serum ferritin level but not with age, sex, Body Mass Index (BMI) and duration of transfusion therapy. The majority of Splenectomised (SPL) patients had restrictive pattern. Only 5 patients had positive prick test which shows that BTM did not relate to their positive prick test. Cell, Gene and Therapy, Vol.1, Number 1, April 1st, 2019; 64-72